Effects of triazophos on biochemical substances of transgenic. Jan 18, 2018 the brown planthopper bph, nilaparvata lugens stal, is the most economically important rice pest throughout asian countries. Reference genes for quantitative realtime pcr analysis in. Susceptibility to neonicotinoids and risk of resistance. Silencing a sugar transporter gene reduces growth and. Nov 19, 2019 biological notes and effect of insecticides on the water measurer bug, hydrometra sp. Nilaparvata is a crucial genus economically, with one amongst its member n. Nitrogen n limitation is well documented for the brown planthopper bph nilaparvata lugens stal, but phosphorus p and potassium k limitation is poorly studied. It also has been observed feeding on rice flower pollen. Nilaparvata lugens an overview sciencedirect topics.
Seasonal abundance of macropterous nilaparvata lugens and. Glutathione stransferases as antioxidant defence agents. Pdf taxonomy and biology of nilaparvata lugens hom. The color of compound eyes is an important biological characteristic of insects.
Nilaparvata lugens elevated co 2 biochemical substances control ef. An nilaparvata lugens in nahilalakip ha genus nga nilaparvata, ngan familia nga delphacidae. The migration of nilaparvata lugens stal delphacidae and. Biological notes and effect of insecticides on the water measurer bug, hydrometra sp. Effects of cry1ab toxin on propylea japonica thunberg. Buprofezin susceptibility survey, resistance selection and. The influence of down regulating expression of sugar transporter genes on insect growth. Nilaparvata lugens adults were collected from insecticide free rice fields located on farms of zhejiang university. Screening and functional analyses of nilaparvata lugens. The influence of down regulating expression of sugar transporter genes on insect growth, development, and fecundity is unknown.
Read seasonal abundance of macropterous nilaparvata lugens and sogatella furcifera based on presumptive macroptery in fifth. Effects of triazophos on biochemical substances of. Biochemical bases of resistance to brown planthopper nilaparvata lugens stal in different rice accessions k. The genetic inheritance and physiological effect of the eye color mutation in the bph have been studied, but the location of. Bacterial symbionts of the brown planthopper, nilaparvata. Chromosomal location of a recessive redeye mutant gene in.
Experiments indicated that the effects of the tested pesticides were dependent on nymphal age, pesticide and their dose and time after application. Adaptation of the brown planthopper, nilaparvata lugens stal, to. The brown planthopper nilaparvata lugens is one of the most serious rice plant pests in. Delphacidae, susceptibility of the treated rice plants and amounts of free amino acids and sucrose were studied.
There have also been many reports of a yeastlike symbiont of n. Insectfree rice plants were used in the following tests at the. In this study, the modified bulk seedling test method was used to identify the bph resistance level and host feeding choice of. A nicotinic acetylcholine receptor mutation conferring. Feedinginduced intra and interspecific interactions. Nilaparavata lugens stal genetic divergence analysis. Studies on host plant resistance to brown planthopper nilaparvata lugens stal. Identification of a novel locus, bph38t, conferring. A series of laboratory experiments was conducted using electrical penetration graph, salivary flange, and honeydew measurement to study the effects of feedinginduced intra and interspecific interactions on feeding behavior and honeydew excretion between planthoppers nilaparvata lugens stal and sogatela furcifera horvath. Currently, chemical control is still a major method for suppressing n.
In contrast, the brown plant hopper nilaparvata lugens uses a different mechanism to overcome sieve tube callose. As with other classes of insecticides, resistance to neonicotinoids is a significant threat and has. Results results indicated that substantial variations in the susceptibility to different insecticides. The free yls in the hemolymph then move to the ovarioles near the pedicel. Ecological fitness of brown planthopper, nilaparvata lugens stal, to. We studied the effects of n, p, and k application on chemical composition of rice plants and its consequences on life parametersadult longevity, fecundity, and egg hatchability of bph. Feedinginduced interactions between two rice planthoppers.
As with other classes of insecticides, resistance to neonicotinoids is a significant threat. Complete nucleotide sequence of the nilaparvata lugens. Insects free fulltext transcriptomic analysis of the. Phloem sap samples of resistant and susceptible rice plants were screened for uv lightabsorbing compounds by means of high performance liquid chromatography hplc. Delphacidae, is an economically important insect of rice in asia heinrich, 1994. Susceptibility to insecticides and ecological fitness in.
In this study, our purpose was to reveal the components and functions of saliva in a rice sapsucking insect pest, nilaparvata lugens. The accomplishment of the whole genome and transcriptome sequencing in n. The nymphs showed an obvious moulting defect phenotype, lower survival rate and significantly reduced chitin content after mir. Although nilaparvata seminula should be reexamined, 14 determined and 2 undetermined species are known as the members of the genus nilaparvata. Paris of rice varieties carrying the bph1, bph2 and bph3 resistance genes are compared as hosts for n. Most phloemfeeding insects secrete gelling and watery saliva during the feeding process. Murugesan department of agricultural entomology, agricultural college and research institute, killikulam, vallanad628 252, india. Delphacidae is a planthopper species that feeds on rice plants oryza sativa l. Ecological fitness of brown planthopper, nilaparvata lugens stal, to rice nutrient management md. The brown planthopper, nilaparvata lugens, is the most serious pest of the rice plant in east and southeast asia, because of the severe damage it causes by sucking sap and transmission of viruses to the rice plant.
Nilaparvata lugens, sogatella furczfera, egg parasitoid, anagrus, oligosita. Antibiosis effects of rice carrying bph14 and bph15 on the. The brown planthopper bph, nilaparvata lugens is a devastating rice insect. The migration of nilaparvata lugens stal delphacidae and other hemiptera associated with rice during the dry season in the philippines.
The feeding responses, growth and population development of nilaparvata lugens stal are quantified on a range of twelve susceptible or resistant hostplants. Jun 25, 2001 selection of a laboratory colony of the brown planthopper nilaparvata lugens with the pyrethroids permethrin and. The complete mitochondrial genome of nilaparvata lugens stal, 1854 captured in korea hemiptera. The brown planthopper bph, nilaparvata lugens stal hemiptera. Pymetrozine activates trpv channels of brown planthopper. Media in category nilaparvata lugens the following 2 files are in this category, out of 2 total. Nilaparvata lugens is one of the major pests of rice and results in substantial yield loss every year. In the present study, we further examined the transcriptome of n. Beetles are abundant in ricefields and could help suppress populations of several insect pests. Blackwell publishing ltd dynamics of imidacloprid resistance. Oct 10, 2019 among these insect pests, brown planthopper bph. All structured data from the file and property namespaces is available under the creative commons cc0 license. Biochemical analysis and synergistic studies with metabolic inhibitors indicated that elevated glutathione stransferases gsts with a predominant peroxidase activity conferred resistance to both pyrethroids.
However, little is known about the diversity of the symbiotic fungal ylss that are harbored in the eggs of bphs and the difference between fertilized eggs and nonfertilized eggs. However, the functions of salivary proteins are poorly understood. The complete mitochondrial genome of nilaparvata lugens. In this study, we examined the bacterial microbes in n. Neonicotinoids, such as imidacloprid, are nicotinic acetylcholine receptor nachr agonists with potent insecticidal activity. Buprofezin susceptibility survey, resistance selection and preliminary determination of the resistance mechanism in nilaparvata lugens homoptera. Nilaparvata lugens is generally called as brown plant hopper or asian rice brown plant hopper bph.
Response of adult brown planthopper nilaparvata lugens. Our previous study found that the entomopathogenic fungus metarhizium anisopliae showed effective potential for controlling this pest. Selection of a laboratory colony of the brown planthopper nilaparvata lugens with the pyrethroids permethrin and. The brown planthopper bph, nilaparvata lugens, is the most destructive insect pest in rice production worldwide. In the laboratory, adults were released in drum cages 20 cm diameter, 75 cm long containing booting plants of kmd1, kmd2, or nontransgenic xs11, and maintained in the chamber as described above. The delphacid genus nilaparvata consists of 18 species.
Resistance to nilaparvata lugens in rice lines introgressed with the. We used a mapping population of 1 doubledhaploid lines, produced from a cross between an improved indica rice variety ir64 and a traditional japonica variety azucena, to detect quantitative trait loci qtls for resistance to the brown planthopper bph, nilaparvata lugens. We evaluated the parents and mapping population with six tests that measure varying combinations of the. One end of the egg is united near the egg caps, the other remains free. An nilaparvata lugens in uska species han insecta nga syahan ginhulagway ni stsl hadton 1854. The virus was found in the cytoplasm of the insect cells, sometimes associated with tubular structures, which is one of the characteristic features. Nitrogen recycling in the brown planthopper, nilaparvata lugens. Here we combined 2de comparative proteomic and rnaseq transcriptomic analyses to identify fecundityrelated proteins and genes. May 04, 2016 most phloemfeeding insects secrete gelling and watery saliva during the feeding process. These insects are among the most important pests of rice, which is the major staple crop for about half the worlds population. Detection and analysis of qtls for resistance to the brown. The virus was found in the cytoplasm of the insect cells, sometimes associated with tubular structures, which is one of the characteristic features in tissues infected with reoviruses. Files are available under licenses specified on their description page. Oct 02, 20 as an rstrategy insect species, the brown planthopper bph nilaparvata lugens stal is a serious pest of rice crops in the temperate and tropical regions of asia and australia, which may be due to its robust fecundity.
Ecological fitness of brown planthopper, nilaparvata. Aug 01, 2006 nilaparvata lugens adults were collected from insecticide free rice fields located on farms of zhejiang university. The planthopper transmits two kinds of viruses, rice ragged. Feeding responses of the brown planthopper, nilaparvata.
Bph causes significant damage to the rice crop by sucking sap from the xylem and phloem tissues, which ultimately leads to hopperburn and. Heong3, lu zhongxian1 1state key laboratory breeding base for zhejiang sustainable pest and disease control, institute of plant protection and. We studied the effects of n, p, and k application on chemical composition of rice plants and its consequences on life parametersadult longevity, fecundity, and egg. Pdf the brown planthopper bph nilaparvata lugens transmits both rice grassy. The genome of nlrv consists of 28 699 nucleotides and contains at least 11 large open reading frames orfs. The brown planthopper nilaparvata lugens stal, the most destructive pest of rice, has been identified, including biotypes with high virulence towards previously resistant rice varieties. Proteomic and transcriptomic analyses of fecundity in the. Parasitic activities of egg parasitoids on the rice planthoppers. Delphacidae, is one of the most harmful insect pests of rice plants, not only directly sucking the phloem sap but also transmitting diseases to host plants as a viral vector cuong et al.
Response of adult brown planthopper nilaparvata lugens stal. A serious pest of paddy, the brown planthopper nilaparvata lugens. Delphacidae yanhua wang, 1,2 congfen gao, zhiping xu, yu cheng zhu,3. Jiushuang zhang,1 wenhong li, 1dejiang dai, youwei lin, weijun zhou,1 and jinliang shen. Delphacidae population free from insecticides in the laboratory dong biqin1, 2, yang yajun1, xu hongxing1, zheng xusong1, k. Since its introduction in the early 1990s, imidacloprid has become one of the most extensively used insecticides for both crop protection and animal health applications. The development and cultivation of bphresistant varieties is the most economical and efficient strategy to overcome the destruction caused by bph. We evaluated the parents and mapping population with six tests that measure varying combinations of the three. Resistant rice varieties of a field nilaparvata lugens stal hemiptera. The nucleotide sequences of all genome segments of the nilaparvata lugens reovirus nlrv, which is found in the brown planthopper nilaparvata lugens, have been determined and some genes have been assigned to structural and functional proteins. Biochemical analysis and synergistic studies with metabolic inhibitors indicated that elevated glutathione stransferases gsts with a predominant peroxidase activity conferred resistance to both pyrethroids, whereas. Delphacidae, a serious insect pest of rice in tropical and temperate asia. Delphacidae is a major rice pest that harbors an endosymbiont ascomycete fungus, entomomyces delphacidicola str. The brown planthopper bph, nilaparvata lugens, sugar transporter gene 6 nlst6 is a facilitative glucosefructose transporter often called a passive carrier expressed in midgut that mediates sugar transport from the midgut lumen to hemolymph.
The objective of this study was to determine the susceptibility of n. The biochemical basis of resistance of rice plants to the brown planthopper, nilaparvata lugens, was investigated. Its outbreaks constitute a serious threat to the sustainability of. Ecological fitness of brown planthopper, nilaparvata lugens. Jun 14, 2005 neonicotinoids, such as imidacloprid, are nicotinic acetylcholine receptor nachr agonists with potent insecticidal activity.
Pdf rice viruses transmitted by the brown planthopper. The brown planthopper bph nilaparvata lugens is an economically important pest of rice in asia. Pdf the complete mitochondrial genome of nilaparvata lugens. A nicotinic acetylcholine receptor mutation conferring target.
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